组织芯片(tissue microarray,TMA)的概念由Kononen等[1]在1998年首次提出,是在一个蜡块上排列数百例组织标本,通过一张切片同时检测数百例组织的基因及蛋白表达。因此,组织芯片作为一种高通量、大样本的分子分析工具广泛应用于医学研究中。但是在利用组织芯片检测肿瘤生物学标记物的过程中,由于制作所用的组织小,直径仅2mm,且同一患者的肿瘤细胞之间存在异质性,因此组织芯片检测肿瘤标记物结果的可靠性尚有待进一步验证。为此,本文通过对比组织芯片与常规石蜡切片在检测胰腺癌组织中 The concept of tissue microarray (TMA), first proposed by Kononen et al [1] in 1998, is to arrange hundreds of tissue samples on a wax block and to simultaneously examine the gene and protein expression of hundreds of tissues by a single slice . Therefore, tissue microarrays are widely used in medical research as a high-throughput, large sample molecular analysis tool. However, in the process of detecting tumor biomarkers using tissue microarrays, the tissue microarray detects the reliability of tumor marker results due to the fact that the tissue used in the fabrication is small and only 2 mm in diameter, and heterogeneity exists between the tumor cells of the same patient Still to be further verified. To this end, this article by comparing tissue and conventional paraffin sections in the detection of pancreatic cancer