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目的:采用一种全新的条件性位置偏爱装置,探索一种可以使动物同时产生奖赏和厌恶记忆的实验模型。方法:使用一种全新的条件性位置偏爱箱,其独特的“Y”字形结构具有三个方向的开口,可以使动物向三个方向进行探索,同时进行两种药物的训练。采用大鼠蔗糖-奎宁自然奖赏和厌恶、甲基苯丙胺-氯化锂以及吗啡-氯化锂药物奖赏和厌恶同时建立动物的条件性位置偏爱以及偏恶模型。结果:(1)在蔗糖-奎宁试验中,大鼠在伴蔗糖箱中的停留时间明显高于伴去离子水箱(P<0.05),在伴奎宁箱中停留时间明显低于伴去离子水箱(P<0.01)。(2)在甲基苯丙胺-氯化锂试验中,大鼠在甲基苯丙胺伴药箱停留时间明显高于生理盐水伴药箱(P<0.05),大鼠在氯化锂伴药箱停留时间明显低于生理盐水伴药箱(P<0.05)。(3)在大鼠吗啡-氯化锂条件位置偏爱实验中,大鼠在吗啡伴药箱停留时间明显高于生理盐水伴药箱(P<0.01),在氯化锂伴药箱停留时间明显低于生理盐水伴药箱(P<0.001)。结论:该新型实验装置可以使动物同时产生条件性位置偏爱和位置偏恶,可以建立动物同时具有奖赏记忆和厌恶记忆的实验模型。
OBJECTIVE: To explore an experimental model that allows animals to generate both rewards and aversive memories using a new conditional place preference device. Method: Using a brand new conditional position preference box, its unique “Y” glyph has three directional openings that allow animals to explore in three directions while training for both drugs. Conditional preference and dysthymia models were established simultaneously with rat sucrose-quinine natural reward and distaste, methamphetamine-lithium chloride, and morphine-lithium chloride drug reward and distaste. Results: (1) In the sucrose-quinine test, the residence time of rats in sucrose-containing tank was significantly higher than that in deionized water tank (P <0.05), and the retention time in quinine tank was significantly lower than that in deionized water Water tank (P <0.01). (2) In the methamphetamine-lithium chloride test, the retention time of rats in the methamphetamines with the medicine box was significantly higher than that of the saline with medicine boxes (P <0.05) Significantly lower than the saline with the chest (P <0.05). (3) In morphine - lithium chloride condition preference experiment, the retention time of rats in morphine - accompanying medicine box was significantly higher than that in saline - medicine cabinet (P <0.01), and the retention time in lithium chloride accompanying medicine box was obviously Lower than saline with kit (P <0.001). Conclusion: The new experimental device can make the animal produce conditional preference and position deviation at the same time, and establish an animal model with reward memory and aversion memory.