The process of cold acclimation is an important adaptive response whereby many plants from temperate regions increase their freezing tolerance after being exposed to low non-freezing temperatures.The correct development of this response relies on proper accumulation of a number of transcription factors that regulate expression pattes of cold-responsive genes.Multiple studies have revealed a variety of molecular mechanisms involved in promoting the accumulation of these transcription factors.Interestingly,however,the mechanisms implicated in controlling such accumulation to ensure their adequate levels remain largely unknown.In this work,we demonstrate that prefoldins (PFDs) control the levels of HY5,an Arabidopsis transcription factor with a key role in cold acclimation by activating anthocyanin biosynthesis,in response to low temperature.Our results show that,under cold conditions,PFDs accumulate into the nucleus through a DELLA-dependent mechanism,where they interact with HY5,triggering its ubiquitination and subsequent degradation.The degradation of HY5 would result,in tu,in anthocyanin biosynthesis attenuation,ensuring the accurate development of cold acclimation.These findings uncover an unanticipated nuclear function for PFDs in plant responses to abiotic stresses.