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流式细胞光度术(简称FCM)是70年代发展起来的新技术,其优点是快速而有效地测定单个细胞的DNA含量。近来有关治疗效果的观察和判断预后的报告较多,而应用于诊断价值的研究报告甚少。本文应用美制FACS420型流式细胞光度仪测定34例宫颈脱落细胞DNA含量,并分成下列三组与细胞学及病理学比较分析,得出下列结果:Ⅰ组8例(巴氏Ⅰ—Ⅱ级),包括正常宫颈和轻度宫颈炎症,其细胞增殖指数是7.9％;Ⅱ组21例(巴氏Ⅲ级),包括重度宫颈炎症和伴有不典型增生,其细胞增殖指数是18.5％;Ⅲ组5例(巴氏Ⅳ—Ⅴ级),病理诊断均为宫颈鳞状细胞癌,其细胞增殖指数是51.4％。三组的数据在统计学上有明显差异,说明FCM测定DNA含量有可能成为子宫颈癌早期诊断中解决疑难问题的一条新途径。
Flow cytometry (FCM) is a new technology developed in the 1970s, which has the advantage of rapidly and effectively determining the DNA content of a single cell. Recently, there have been more reports on the observation of therapeutic effects and prognosis of judgments, and few reports have been reported on the diagnostic value. In this paper, 34 cases of cervical exfoliated cells DNA content were determined by FACS420 flow cytometer in the United States, and divided into the following three groups with cytology and pathology analysis, the following results: Ⅰ group of 8 patients (Pasteur I-Ⅱ grade) , Including normal cervical and mild cervical inflammation, the cell proliferation index was 7.9%; Ⅱ group 21 cases (grade Ⅲ), including severe cervical inflammation and accompanied by atypical hyperplasia, the cell proliferation index was 18.5%; Ⅲ group 5 cases (grade Ⅳ-Ⅴ), pathological diagnosis of cervical squamous cell carcinoma, the cell proliferation index was 51.4%. Statistically significant difference between the three groups of data, indicating that the determination of DNA content by FCM may become a new way to solve difficult problems in the early diagnosis of cervical cancer.