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抗癌中药镇痛宝具有活血化淤、消退肿块、消除剧烈疼痛等功效。为了进一步观察其抗癌作用,参照“抗肿瘤药物药效指导原则”,以及“治疗恶性肿瘤中药的药效学研究”等要求进行本实验研究。 1 材料和方法 1.1 动物:昆明系雄性小鼠,日龄28～30天,体重18～22g,北京医科大学实验动物科学部提供。该科学部取得北京市实验动物管理委员会一级合格证书。 1.2 癌株:可移植性H_(22)肝癌细胞株,腹水型。由北京肿瘤防治所药理研究室提供。每7日腹腔注射传代一次。实验用传代第7天的荷瘤小鼠,无菌抽出腹水用生理盐水稀释成瘤细胞浓度5×10~7/ml,冰浴,备用。
Anti-cancer Chinese medicine Analgesia has functions of promoting blood circulation, removing masses, and eliminating severe pain. In order to further observe its anti-cancer effects, refer to the “Guiding Principles of Antitumor Drug Effectiveness” and “Requirements for Pharmacodynamic Study of Treating Malignant Tumors” and other requirements for this experimental study. 1 Materials and methods 1.1 Animals: Kunming male mice, 28 to 30 days old, weighing 18 to 22 g, provided by the Laboratory Animal Science Department of Beijing Medical University. The Ministry of Science has obtained the Class 1 certificate from the Beijing Experimental Animal Management Committee. 1.2 cancerous lines: transplantable H_(22) liver cancer cell lines, ascites type. Provided by the Pharmacology Laboratory of the Beijing Cancer Institute. Intraperitoneal injections were performed once every 7 days. The experiment was carried out on the 7th day of the passage of the tumor-bearing mice. The ascites was aseptically diluted with saline to produce a tumor cell concentration of 5×10~7/ml, ice bathed and used.