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目的:探索蔓龙胆醇提物对小鼠急性酒精性肝损伤的保护作用及其安全性评价。方法:取昆明种小鼠60只,随机分为6组:正常对照组,模型对照组,蔓龙胆醇提物低、中、高剂量组(2.5、5、10 g/kg),联苯双酯阳性对照组0.2 g/kg;灌胃给药,每日1次,每周称一次体重,连续20 d,末次灌胃2 h后,除正常对照组灌等量蒸馏水外,其余各组灌服50%酒精12 ml/kg,禁食不禁水16 h后处死,按照试剂盒检测血清中谷天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、肝匀浆液总蛋白、谷胱甘肽(GSH)、丙二醛(MDA)。取昆明种小鼠40只,分为对照组和给药组,以最大给药浓度和最大给药容量灌胃给药,连续观察15 d后,测定其最大给药量倍数。结果:与模型对照组相比,蔓龙胆醇提物给药组可以降低血清AST、TG和肝匀浆MDA;给药组小鼠的最大给药倍数相当于成人临床用量428.8倍,安全性高。结论:蔓龙胆醇提物对酒精致肝损伤具有一定的保护作用,临床拟用药量安全。
Objective: To explore the protective effect of Gendorangium alcohol extract on mice with acute alcoholic liver injury and its safety evaluation. Methods: Sixty Kunming mice were randomly divided into 6 groups: normal control group, model control group, gentian alcohol extract low, middle and high dose group (2.5, 5, 10 g / kg), biphenyl D-galactose positive control group 0.2 g / kg; gavage administration, once daily, weighed once a week for 20 days, the last 2 hours after gavage, in addition to the normal control group, the same amount of distilled water, the other groups The rats were sacrificed after 16 hours of fasting with 50% ethanol (12 ml / kg). The contents of aspartate aminotransferase (AST), triglyceride (TG), total protein in liver homogenate, Glutathione (GSH), malondialdehyde (MDA). Forty Kunming mice were randomly divided into control group and treatment group. The rats were administered with the maximum drug concentration and the maximum drug delivery capacity. After 15 days of continuous observation, the maximum dose was determined. Results: Compared with the model control group, gentian alcohol extract administration group could reduce serum AST, TG and liver homogenate MDA; the maximum administration multiple of the mice in the administration group was equivalent to 428.8 times of the clinical dosage in adults, and the safety high. Conclusion: Gendan gall alcohol extract on alcohol-induced liver injury has a protective effect, the clinical dosage to be safe.