论文部分内容阅读
We examined the effects of calcitonin gene-related peptide CGRP on the bone resorption stimulated by IL-1χ after neonatal mouse calvaria were culatured in vitro and bone resorption was determined by measuring the release of calcium into the medium after χ 48-hour incubation. CGRP (10~(-9)-10~(-8) M) blocked completely the IL1χ-mediated increase in bone resorption and inhibited the basal bone resorption. In accordence with the effects of calcitonin on osteoclasts, CGRP caused cessation of lamellipodial activity and gradual retraction of lamellipodia when added into the culture medium.Quantitatitive analysis showed that CGRP (10~(-6) M) caused a gradual reduction of osteoclastic surface area and convex perium. These data show that CGRP inhibits IL1χ mediated bone resorption because of its direct regulation of osteoclast activity.
We examined the effects of calcitonin gene-related peptide CGRP on the bone resorption stimulated by IL-1χ after neonatal mouse calvaria were culatured in vitro and bone resorption was determined by measuring the release of calcium into the medium after χ 48-hour incubation. CGRP (10 ~ (-9) -10 ~ (-8) M) blocked completely the IL1χ-mediated increase in bone resorption and inhibited the basal bone resorption. In accordence with the effects of calcitonin on osteoclasts, CGRP caused cessation of lamellipodial activity and gradual retraction of lamellipodia when added into the culture medium. Quantitatitive analysis showed that CGRP (10 ~ (-6) M) caused a gradual reduction of osteoclastic surface area and convex perium. These data show that CGRP inhibits IL1χ mediated bone resorption due to its direct regulation of osteoclast activity.