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本文作者建立定量RT-PCR方法。探讨iceA体内表达量与临床和组织病理学、其他幽门螺杆菌(Hp)毒力相关基因及iceA基因结构的关系。选择41例Hp阳性患者,在其胃大弯部距幽门3～5cm进行活检,活检组织分别用于组织学检查、细菌培养及定量RT-PCR。在进行定量RT-PCR时,先提取标本的总RNA,利用随机六聚体引物反转录合成cDNA。然后设计了3对引物并用生物素标记,分别用于扩增iceA1、iceA2和16s rRNA。每个PCR
The authors establish a quantitative RT-PCR method. To investigate the relationship between the expression level of iceA and the clinical and histopathological features, the virulence related genes of other Helicobacter pylori (Hp) and the iceA gene structure. 41 patients with positive Hp were selected for biopsy at 3 ~ 5cm of pyloric pachyna. The biopsy tissues were used for histological examination, bacterial culture and quantitative RT-PCR, respectively. For quantitative RT-PCR, total RNA was extracted from the samples and cDNA was reverse transcribed using random hexamer primers. Three pairs of primers were designed and labeled with biotin for amplification of iceA1, iceA2 and 16S rRNA, respectively. Each PCR