作者结合自己科研实践进行文献综述,阐述了内标和载体的选择与应用等重要问题。用色谱法(TLC、HPLC、GC或GC-MS)测定体液样品中药物含量前,加入内标后再行测定,用药物与内标色谱响应值(峰高或峰面积)之比值代替药物绝对响应值按内标法定量,可增加结果的精密度和准确度,简化操作,此法尤适于体液样品中药物用GC作定量分析。内标选择是本法的 The author summarizes the literature with his research practice, and expounds the important issues such as the selection and application of internal standard and vector. Before the determination of the drug content in a body fluid sample by chromatography (TLC, HPLC, GC or GC-MS), add the internal standard before proceeding with the determination of the ratio of drug to internal standard chromatographic response (peak height or peak area) The response value is quantified by internal standard method, which can increase the precision and accuracy of the results and simplify the operation. This method is especially suitable for the quantitative analysis of drugs in body fluid samples by GC. Internal standard selection is the law