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本试验对月季实生苗进行了试管快速繁殖和移栽研究。初代培养各种激素试验中,MS+BA2+GA10的培养基较好,本培养基外植体萌发快,增殖高达8~10倍。47代培养中产生的细弱苗,转移到壮苗培养基MS+BA0.5+NAA0.5中培养2周,壮苗移植在诱导根原基的MS+NAA0.5的培养基中培养,经约两周左右培养产生根原基。然后,将它移植于生根培养基上,生根培养基本为1/2MS+NAA0.5+300mg/l活性碳,强光条件下,温度白大控制在28℃,夜间控制在21℃左右。生根后移栽盆土为腐殖士:河砂:锯沫=1:1:1。盖上烧杯封闭一周后,开始每天提烧杯0.5cm,3天后,方可移我在花盆中的腐殖土上培养。
In this experiment, rapid growth of test tubes and transplanting of rose seedlings were studied. Primary culture of various hormones in the test, MS + BA2 + GA10 better medium, the medium of explants germination fast, up to 8 to 10 times the proliferation. 47 generations of seedlings generated in culture, transferred to strong seedling medium MS + BA0.5 + NAA0.5 for 2 weeks, strong seedling transplantation rooted in the induction of MS + NAA0.5 medium, after about two weeks of culture to produce root base. Then, it was transplanted on the rooting medium, rooting culture is basically 1 / 2MS + NAA0.5 +300 mg / l activated carbon, under light conditions, the temperature of white control at 28 ℃, night control at about 21 ℃. Rooted after transplanting pots for the humus: river sand: sawdust = 1: 1: 1. Close the beaker for one week, then start a beaker 0.5cm daily, 3 days before I can be moved in the humus soil culture.