论文部分内容阅读
用新研制的维甲酸的衍生物维胺酸作为分化诱导剂。以上皮细胞来源的癌细胞,如小鼠前胃癌细胞MFC(转移率为85％)、人鼻咽癌细胞系CNE-2Z(转移率为56％)、CNE-2Z的亚系克隆株L2(高转移细胞株,转移率100％)以及L4(低转移细胞株,转移率为13％)作为研究对象,用体外培养的癌细胞,以10~(-5)mol/LRⅡ作用5代后,进行了系列观察。实验结果显示,RⅡ作用于MFC细胞和CNE-2Z及其克隆株后,在生长曲线、集落形成、运动性及侵袭性均查见有明显抑制作用,但对FN和LN基质的粘附性均有升高;在形态下也出现明显的微细结构的改变,主要为细胞表面的改变。这些改变说明RⅡ有一定分化诱导作用,使癌细胞有向正常分化的趋向性。对癌基因表达的影响,动物癌细胞及人癌细胞受RⅡ作用后,与转移的相关性有明显矛盾现象。如高转移的MFC细胞对nm~(23)不表达,RⅡ作用后出现表达而CNE-2Z克隆株中高转移细胞中的nm~(23)表达,在RⅡ作用后受到抑制。在CNE-2Z及其克隆株中发现fos,ras~H,nm和Rb4个基因的表达,具有共同特点。RⅡ作用后在母系及高转移的L2细胞株中的表达受抑制,而在低转移L4细胞株中均呈高表达。提示癌基因、抑癌基因在不同侵袭转移性癌细胞中引起的作用可能不尽相同,同时对RⅡ作用后不同癌细胞中其基因表达也不尽相同。因此我们认为
A newly developed retinoid derivative, vetine, was used as a differentiation inducer. The above skin cell-derived cancer cells, such as mouse MFC (metastasis rate 85%), human nasopharyngeal carcinoma cell line CNE-2Z (metastatic rate 56%), CNE-2Z subline clonal strain L2 ( High-metastatic cell line (100% metastasis rate) and L4 (low metastatic cell line, metastasis rate of 13%) were used as research subjects. After in vitro cultured cancer cells were treated with 10~(-5) mol/LRII for 5 passages, A series of observations was made. The experimental results showed that after RII was applied to MFC cells and CNE-2Z and its clones, the growth curve, colony formation, motility and invasiveness were all found to have significant inhibitory effects, but the adhesion to FN and LN matrices were both There is an increase; there are also obvious changes in the micro structure under the morphology, mainly for cell surface changes. These changes suggest that RII has a certain differentiation-inducing effect, leading to the tendency of cancer cells to differentiate normally. The influence of oncogene expression, animal cancer cells and human cancer cells by RII, there is a clear contradiction between the correlation with metastasis. For example, high-metastasis MFC cells do not express to nm23, but RII expression and nm23 neutropenia expression in CNE-2Z clones are inhibited by RII. The expressions of fos, ras~H, nm and Rb genes in CNE-2Z and its clones were found to have common features. The expression of RII in the maternal line and the highly metastatic L2 cell line was inhibited, while it was highly expressed in the low metastatic L4 cell line. The role of oncogenes and tumor suppressor genes in different invasion and metastasis cancer cells may be different. At the same time, the gene expression in different cancer cells after RII action is also not the same. So we think