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为明确运用脉动真空蒸汽灭菌 ,对凡士林纱布的灭菌是否安全有效及油纱布厚度是否影响灭菌效果。将凡士林纱布按层数不同分为四组 ,每组样本 3 0例 ,用三种监测方法 :1 )留点温度计 ;2 )化学监测 :1 3 2°C压力蒸汽灭菌化学指示卡 ;3 )生物监测 :嗜热脂肪杆菌芽孢菌片。经过 3 0次灭菌试验得出结果 :1 )凡士林纱布越厚 ,蒸汽越不易穿透 ,其所得到的温度越低 ,化学指示卡的变色浅而不匀 ;2 )凡士林纱布厚度超过 60层 ,菌片培养出现阳性 ;3 )凡士林纱布的厚度控制在 60层以内 ,运用脉动真空蒸汽灭菌 ,其效果安全可靠。
To clarify the use of pulsating vacuum steam sterilization, Vaseline gauze sterilization is safe and effective and gauze thickness affect the sterilization effect. The Vaseline gauze is divided into four groups according to different layers, 30 samples in each group, using three monitoring methods: 1) stay point thermometer; 2) chemical monitoring: 1 3 2 ° C pressure steam sterilization chemical indicator; 3 Biomonitoring: Bacillus stearothermophilus tablets. After 30 sterilization tests, the results are as follows: 1) The thicker Vaseline gauze is, the more difficult the steam is to penetrate. The lower the temperature obtained, the lighter and less uneven the chemical indicator card. 2) The thickness of Vaseline gauze exceeds 60 layers , Bacterial culture positive; 3) Vaseline gauze thickness control within 60 layers, the use of pulsed vacuum steam sterilization, the effect of safe and reliable.