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在研究肝糖元储存和消耗分布区域的基础上,我们用萤光显微镜探讨了肝脏RNA与PAS染色的糖元分布的相互关系。结果证明在消耗型者,肝小叶内糖元与RNA分布相反,即糖元仅留于中中央区,而RNA呈现于门管区。在储存型二者均显示门管型。糖元全部消失者,RNA还不完全消失。切片标本经甲綠——派郎宁、甲苯胺蓝,棓酸天青蓝三种组织化学染色,光学显微镜下所显示肝小叶RNA的分布,与螢光镜像的结果一致。利用螢光显微镜技术作为观察肝脏 RNA分布的指标,是具有理论和实际意义的。如与其他常规组织化学染色方法相比较,则螢光镜技术显示出了指标可靠、准确及方法简便等较大的优越性。
Based on the study of liver glycogen storage and depletion areas, we used fluorescence microscopy to investigate the relationship between liver RNA and PAS-stained glycogen distribution. The results showed that in the consumptive type, the distribution of glycogen and RNA in the hepatic lobule was opposite, that is, the glycogen remained only in the central region, while the RNA was present in the portal area. Both of the storage models show door type. When all glycogens disappear, RNA does not completely disappear. Specimens were stained with Ala-Green-Pranine, Toluidine Blue, and Citrate Blue, and the distribution of RNA in the hepatic lobule revealed by light microscopy was consistent with the fluorescent mirror image. The use of fluorescent microscopy as an indicator of the distribution of liver RNA is of theoretical and practical significance. If compared with other conventional tissue chemical staining methods, the fluoroscopy technology shows that the index is reliable, accurate and simple.