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本文报道了坛紫菜叶状体经60Coγ射线辐照后,用酶解方法获得离体细胞,观察了体细胞的分裂分化及进行单克隆培养叶状体.结果显示:体细胞发育和分化的趋势分为正常叶状体、畸形叶状体、细胞团、丝状体和根丝细胞5种方式;通过单克隆的培养,初步筛选出1个抗逆性较强、生长速度较快的新品系D,该品系1m以上长度的叶状体平均生长速度为9.4cm/d,快的可达11.5cm/d.对新品系的子一代叶状体再度酶解和观察,获得纯系的丝状体.
In this paper, we report that after irradiating 60Coγ-ray with Pleurotus eryngii, the isolated cells were obtained by enzymolysis, the differentiation of somatic cells was observed, and the fronds were cultured in monoclone. The results showed that there was a tendency of somatic cell development and differentiation Divided into normal fronds, deformities fronds, cell clusters, filaments and root filament cells in five ways; through the monoclonal culture, initially screened a strong resistance, rapid growth of new lines D. The average growth rate of the fronds with the length of more than 1m was 9.4cm / d and the rapidity reached 11.5cm / d. The fronds of the new generation of fronds were re-enzymatically hydrolyzed and observed, body.