目的　在建立C6 Wistar胶质瘤大鼠模型的基础上 ,研究大鼠胶质瘤模型的C6胶质瘤细胞的增殖动力学。方法　应用流式细胞仪检测、PCNA免疫组化染色和核仁组织区银染三种方法 ,研究C6胶质瘤细胞的增殖动力学指标 ,并进行相关性分析。结果　 1、肿瘤细胞接种 2周后 ,肿瘤模型建立 ,HE染色见肿瘤细胞增殖活跃 ,各项增殖指标显示该肿瘤增殖活性较强。 2、AgNor面积、AgNOR计数与S期百分比和PCNA指数具有良好的相关性 ,AgNOR面积 (P <0 0 1)比AgNOR计数 (P <0 0 5 )相关性更强 OBJECTIVE: To study the proliferation kinetics of C6 glioma cells in rat glioma model on the basis of rat model of C6 Wistar glioma. Methods Flow cytometry, PCNA immunohistochemical staining and silver staining of the nucleolus area were used to study the kinetics of C6 glioma proliferation and to analyze the correlation. Tumor cells were established 2 weeks after inoculation, HE staining showed active proliferation of tumor cells, proliferation index showed that the tumor proliferation activity is strong. There was a good correlation between AgNor area, AgNOR count and S phase percentage and PCNA index. AgNOR area (P <0 01) was more correlated with AgNOR count (P 0 05)