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目的观察阿托伐他汀对豚鼠实验性变态反应性脑脊髓炎(EAE)外周血单个核细胞分泌细胞因子(CK)能力的影响,探讨其防治EAE的免疫调节机制。方法采用粗制碱性髓鞘蛋白加福氏完全佐剂皮下注射的方法建立EAE模型。将40只豚鼠随机分成4组:正常对照组、EAE对照组、EAE低剂量治疗组和EAE高剂量治疗组,每组10只,造模前5d正常对照组和EAE对照组每天灌喂氯化钠溶液,EAE低剂量、高剂量治疗组分别在造模前5d开始灌喂含阿托伐他汀1mg/(kg·d)、4mg/(kg·d),直至整个实验结束。酶联免疫吸附法测定各组发病高峰期外周血单个核细胞分泌的细胞因子IFN-γ、IL-4、IL-10、TNF-α的水平。结果 EAE对照组外周血单个核细胞分泌IL-4、IL-10的能力较正常对照组明显低(P<0.05),EAE高、低剂量治疗组外周血单个核细胞分泌IL-4、IL-10的能力较EAE对照组明显增强(P<0.05)。EAE对照组外周血单个核细胞分泌IFN-γ、TNF-α的能力较正常对照组明显增强(P<0.05),EAE高、低剂量治疗组外周血单个核细胞分泌IFN-γ、TNF-α的能力较EAE对照组明显减弱(P<0.05)。结论阿托伐他汀防治EAE的免疫机制可能是通过抑制IFN-γ、TNF-α产生及促进IL-4、IL-10产生而发挥。
Objective To investigate the effect of atorvastatin on cytokine (CK) secretion in peripheral blood mononuclear cells of experimental allergic encephalomyelitis (EAE) in guinea pigs and to explore its mechanism of immune regulation of EAE. Methods The EAE model was established by subcutaneous injection of crude basic myelin plus carbofuran complete adjuvant. 40 guinea pigs were randomly divided into 4 groups: normal control group, EAE control group, EAE low-dose treatment group and EAE high-dose treatment group, 10 rats in each group. Normal control group and EAE control group were fed with chloride Sodium and EAE low-dose and high-dose treatment groups were given atorvastatin 1mg / (kg · d), 4mg / (kg · d), respectively, 5d before modeling, until the end of the experiment. The levels of cytokines IFN-γ, IL-4, IL-10 and TNF-α secreted by peripheral blood mononuclear cells were detected by enzyme-linked immunosorbent assay. Results The levels of IL-4 and IL-10 secreted by peripheral blood mononuclear cells in EAE control group were significantly lower than those in normal control group (P <0.05). The levels of IL-4 and IL- 10 than the EAE control group was significantly enhanced (P <0.05). The levels of IFN-γ and TNF-α secreted by peripheral blood mononuclear cells in EAE control group were significantly higher than those in normal control group (P <0.05). The levels of IFN-γ and TNF-α secreted by peripheral blood mononuclear cells Was significantly weaker than EAE control group (P <0.05). Conclusion Atorvastatin may play an important role in the prevention of EAE by inhibiting the production of IFN-γ, TNF-α and promoting the production of IL-4 and IL-10.