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本实验探讨了以羟基磷灰石微量分析法测定大鼠海马内糖皮质激素受体(GR)的方法。实验结果表明:此方法是适用的,而且具有简便快速、节约标记物等特点,用此方法检测人参茎叶皂甙组大鼠海马GR的特异性结合量减少,下降率为48. 2％,而结合力未见明显改变。同时发现人参茎叶皂甙组大鼠海马cAMP含量也下降,推测人参茎叶皂甙海马通过GR和cAMP的变化参入垂体一肾上腺轴的调节。
This experiment explored a method for the determination of glucocorticoid receptor (GR) in rat hippocampus by hydroxyapatite microanalysis. The experimental results show that this method is applicable, and has the characteristics of simple and rapid, saving markers and other methods, using this method to detect the specific binding amount of GR in hippocampus of rats in the ginseng stem and leaf saponin group decreased, the rate of decline was 48.2%. There was no significant change in binding force. At the same time, the content of cAMP in the hippocampus of Ginseng stem and leaf saponin group was also decreased. It was presumed that the changes of Ginsenosides and stem saponins hippocampus into the pituitary-adrenal axis through the changes of GR and cAMP.