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目的通过诱导多能干细胞(i PSCs)技术重编程孤雌胚胎干细胞,探讨i PSCs技术对孤雌胚胎干细胞的多能性及印记基因的影响。方法从孤雌激活的囊胚中建立了孤雌胚胎干细胞;利用反转录病毒将多能性转录因子转入孤雌胚胎干细胞中,建立孤雌i PS细胞。结果建立的孤雌来源的i PS细胞体内外分化能力与孤雌胚胎干细胞的差别无显著性;Real-time PCR结果显示,孤雌i PS细胞母源印记基因的表达明显高于孤雌胚胎干细胞,父源印记基因表达下降,多能性基因表达升高。结论 i PSCs技术能影响基因的表达,尤其是印记基因,印记使其更接近于正常受精来源的胚胎干细胞中印记基因水平。
OBJECTIVE: To reprogram parthenogenetic stem cells by induced pluripotent stem cells (i PSCs) and to explore the effect of i PSCs technology on the pluripotency and imprinting genes of parthenogenetic embryonic stem cells. Methods Parthenogenetic embryonic stem cells were established from parthenogenetic blastocysts. Parthenogenic i PS cells were established by transferring the pluripotency transcription factor into parthenogenetic embryonic stem cells using retrovirus. Results The establishment of parthenogenetic iPS cells in vitro and in vivo differentiation ability and parthenogenetic embryonic stem cells was no significant difference; Real-time PCR results show that Partheno-PS cells maternal imprinting gene expression was significantly higher than parthenogenetic embryonic stem cells , The parental imprinted gene expression decreased, pluripotency gene expression increased. Conclusion The technology of i PSCs can affect the expression of genes, especially the imprinted gene, which makes it closer to the level of imprinted genes in embryonic stem cells of normal fertilization.