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在中枢神经系统微血管形态学研究方面,首次应用钨酸钠(ST)作为稳定剂,以TMB—ST作为成色方法用于过氧化物酶组织化学法显示中枢神经系统微血管构筑。通过对过氧化物酶—TMB—ST法、碱性磷酸酶法(钙铅法)和腺苷三磷酸法8种组织化学法所显示的大鼠脑及脊髓微血管构型的比较观察,以及应用电子计算机图像分析处理系统和光镜下体视学测试系统对微血管的计量学分析比较,表明:过氧化物酶—TMB—ST 法是显示中枢神统微血管的良好方法,具有反应灵敏、产物稳定、方法简便等优点。
In the study of microvascular morphology of central nervous system, the first application of sodium tungstate (ST) as a stabilizer, TMB-ST as a colorimetric method for peroxidase histochemical method of CNS microvascular architecture. By comparing the microvascular configurations of brain and spinal cord demonstrated by 8 histochemical methods of peroxidase-TMB-ST, alkaline phosphatase (calcium-lead method) and adenosine triphosphate method, and the application Computational analysis of microvessels by computerized image processing system and light microscope test showed that peroxydase-TMB-ST method is a good method to display CNS microvessels with sensitive reaction and stable product. Methods Simple and so on.