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收集湖北医科大学口腔医学院15例口腔鳞状细胞癌患者,15例健康人作为对照,运用 HNK_1单克隆抗体和卵白素-生物素-过氧化物酶复合物(ABC)免疫酶染色技术,检测患者外周血和病变局部浸润的 NK 细胞数,观察其分布规律。结果显示:患者外周血 HNK_1~+细胞数明显高于对照组(P<0.01)。外周血 HNK_1~+细胞数量的多少与癌的分级存在着一定的联系;癌组织中 HNK_1~+细胞的分布亦有其规律性。
Fifteen patients with oral squamous cell carcinoma from the School of Stomatology, Hubei Medical University were collected and 15 healthy subjects were used as controls. HNK_1 monoclonal antibody and avidin-biotin-peroxidase complex (ABC) immunoenzyme staining technique were used to detect the disease. The number of NK cells infiltrated in patients’ peripheral blood and lesions was observed and their distribution patterns were observed. The results showed that the number of HNK_1~+ cells in the peripheral blood of patients was significantly higher than that in the control group (P<0.01). The number of HNK_1~+ cells in peripheral blood is related to the grade of cancer; the distribution of HNK_1~+ cells in cancer tissues also has its regularity.