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目的探讨103Pd支架对雄性新西兰白兔尿道组织Bax/Bcl-2基因表达的影响及与细胞凋亡的关系。方法分别将普通支架和103Pd同位素支架置入兔的尿道内,术后30d取尿道标本,采用免疫组化、Tunel方法检测尿道组织Bax/Bcl-2基因表达及细胞凋亡情况。结果放射性支架组尿道组织Bax蛋白表达明显上调,其阳性率为83.3%,明显高于普通支架组的16.7%(P﹤0.05);Bcl-2蛋白表达降低,其阳性率为33.3%,明显低于普通支架组的83.3%(P﹤0.05)。放射性支架组成纤维细胞、平滑肌细胞等凋亡明显,细胞凋亡数为161.93±26.21,尿道无明显狭窄;普通支架组细胞凋亡不明显,细胞凋亡数为65.27±13.39,尿道出现不同程度的狭窄。结论103Pd放射性支架通过增强Bax基因表达、降低Bcl-2基因表达,可促进兔尿道组织细胞凋亡,抑制尿道损伤愈合过程中管腔的再狭窄。
Objective To investigate the effect of 103Pd scaffold on the expression of Bax / Bcl-2 in urethral tissues of male New Zealand white rabbits and its relationship with apoptosis. Methods The common stent and 103Pd isotope stent were placed into the urethra of rabbits respectively. Urethral specimens were taken 30 days after operation. The expression of Bax / Bcl-2 gene and the apoptosis of urethral tissues were detected by immunohistochemistry and Tunel method. Results The positive rate of Bax protein in urethral tissue of radioactive stent group was significantly higher than that of normal stent group (83.3% vs 16.7%, P <0.05). The positive rate of Bcl-2 protein was 33.3% 83.3% of the normal stent group (P <0.05). The apoptosis of fibroblasts and smooth muscle cells in radioactive scaffolds was obvious. The number of apoptotic cells was 161.93 ± 26.21, with no obvious urethral stricture. The apoptosis of normal scaffolds was not obvious, the number of apoptotic cells was 65.27 ± 13.39, narrow. Conclusion The 103Pd radioactive stent can promote the apoptosis of rabbit urethral tissue and inhibit the restenosis of the lumen during the healing of urethral injury by enhancing the expression of Bax gene and decreasing the expression of Bcl-2 gene.