从患典型肠套叠症的濒死斑点叉尾(Ictalurus punctatus)肝脏分离到1株病原菌GX-1,经API 20NE鉴定为温和气单胞菌(Aeromonas sobria)。16S rRNA基因测序结果,该菌株与温和气单胞菌NCIMB 12065株的亲缘关系最近,相似性达99.8%。该菌株对健康斑点叉尾鱼种和成鱼均具有很强的致病力,人工感染发病鱼症状与自然病鱼症状基本一致。药敏试验结果表明,GX-1对头孢拉定、先锋必等15种抗生素敏感,对苯唑青霉素和青霉素G耐药。 One pathogen, GX-1, was isolated from the liver of a moribund Ictalurus punctatus with typical intussusception and identified as Aeromonas sobria by API 20NE. 16S rRNA gene sequencing results showed that this strain had the closest genetic relationship with NCBI 12065 and the similarity was 99.8%. The strain has strong virulence to healthy channel catfish and adult fish, and the symptoms of fish infected by artificial infection are basically the same as the symptoms of natural diseased fish. Susceptibility test results showed that GX-1 cefradine, Pioneer and other 15 kinds of antibiotics will be sensitive to oxacillin and penicillin G resistance.