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为了提高氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans,A.f)的驯化效率,获得能耐受高浓度铀和氟离子的A.f菌株,对分离纯化得到的A.f菌株SKS10的耐铀、耐氟驯化方法进行了研究,提出了连续转接驯化和联合驯化方法.结果表明,连续转接驯化法可加快驯化速度,提高驯化效果,96h可获得氧化速率提高7.5倍、能耐受1000mg/LU6+的菌株,且能获得用传统驯化方法不能得到的耐受1500mg/LU6+的菌株及能耐受120mg/LF-的菌株.与传统驯化法相比,联合驯化法可获得能同时耐受较高铀和氟离子且氧化活性较强的菌株,先进行耐铀驯化再进行耐氟驯化效果更好,在含1000mg/LU6+和80mg/LF-的培养基中,Fe2+的氧化速率是其他驯化方法的6倍以上.
In order to improve the domestication efficiency of Acidithiobacillus ferrooxidans (Af) and obtain the Af strain which can tolerate high concentrations of uranium and fluoride ions, the uranium-and fluoride-tolerant acclimation methods of the isolated and purified Af strain SKS10 were studied , The method of continuous transfer, acclimation and co-acclimation was put forward.The results showed that the continuous transfer and acclimation method can accelerate the domestication speed and improve the domestication effect, and get the strain with a 7.5-fold increase of oxidation rate and 1000mg / LU6 + Compared with the traditional domestication method, the co-acclimation method can obtain a strain that can tolerate higher uranium and fluoride ions at the same time and has a higher oxidation activity than the traditional domestication method Strong strains, the first uranium-resistant domestication and then fluorine-resistant domestication better, with 1000mg / LU6 + and 80mg / LF- medium, Fe2 + oxidation rate of more than 6 times that of other domestication methods.