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目的:探讨用绿色荧光蛋白(GFP)标记的乳腺癌细胞MDA-MB-231建立可量化评估的自发性肺转移和实验性肺转移动物模型,为研究乳腺癌的转移机制提供依据。方法:采用慢病毒载体介导的病毒包装体系,获得稳定表达GFP的细胞系MDA-MB-231-GFP,通过皮下注射方式将细胞接种于Balb/C裸鼠皮下,建立自发性肺转移模型,通过尾静脉注射方式将细胞接种到重症联合免疫缺陷(SCID)小鼠体内,建立实验性肺转移模型,分别于8周和5周后处死小鼠,取肺组织于体视显微镜下观察。结果:自发性肺转移小鼠接种细胞8周后,原位形成直径约为15 mm的肿瘤块;处死小鼠后肺部大体标本未见结节状转移灶,但488 nm激发光下见转移灶呈绿色点状分布。实验性肺转移小鼠接种细胞5周后处死小鼠,肺部形成的转移灶肉眼不可见,但在488 nm激发光波长下同样呈现绿色点状分布。肺转移灶易于观察和统计。结论:成功建立了可以量化的MDA-MB-231细胞肺转移动物模型。
OBJECTIVE: To establish a quantitative and quantitative animal model of lung metastasis and experimental pulmonary metastasis by using green fluorescence protein (GFP) -induced breast cancer cell line MDA-MB-231 to provide evidence for studying the metastatic mechanism of breast cancer. Methods: The lentiviral vector-mediated viral packaging system was used to obtain the cell line MDA-MB-231-GFP stably expressing GFP. The cells were inoculated subcutaneously in Balb / C nude mice by subcutaneous injection to establish a spontaneous lung metastasis model. The cells were inoculated into severe combined immunodeficient (SCID) mice via tail vein injection. Experimental lung metastasis model was established. Mice were sacrificed at 8 weeks and 5 weeks respectively. Lung tissues were observed under stereomicroscope. RESULTS: After 8 weeks of inoculation, the tumor cells were formed in situ in spontaneous lung metastasis mice. After the mice were killed, no nodular metastasis was found in the lung specimens, but the metastasis was seen at 488 nm Kitchen was green dot-like distribution. Mice were sacrificed 5 weeks after inoculation of mice with experimental lung metastasis. The metastatic lesions in the lungs were invisible to the naked eye, but showed a green spot-like distribution at 488 nm excitation wavelength. Lung metastases easy to observe and statistics. Conclusion: The animal model of lung metastasis of MDA-MB-231 cells can be established successfully.