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目的 :建立固定中性粒细胞 - ELISA,分析血清抗中性粒细胞胞浆抗体 (ANCA)对溃疡性结肠炎 (UC)的诊断价值。方法 :采用固定中性粒细胞 - ELISA法检测 2 5份溃疡性结肠炎血清、2 6份细菌性结肠炎血清、1 5份混合类型腹泻血清和 50份正常对照血清中的 ANCA,对各组血清的吸光度 (A值 )进行统计分析。结果 :UC组 ANCA与其它对照组 ANCA之间的差异非常显著(P<0 .0 0 5)。 UC组 A值超过正常对照组 A值 2 s以上病例达 80 % ,诊断特异性达 89%。结论 :UC血清中存在有诊断价值的 ANCA,固定中性粒细胞 - ELISA可作为血清 ANCA检测的重要手段。
Objective: To establish a fixed neutrophil ELISA for the diagnosis of ulcerative colitis (UC) by using serum anti-neutrophil cytoplasmic antibody (ANCA). Methods: ANCA was detected in sera of 25 patients with ulcerative colitis serum, 26 bacterial colitis serum, 15 mixed type diarrhea serum and 50 normal control serum by fixed neutrophil-ELISA. Serum absorbance (A value) for statistical analysis. Results: The difference between ANCA in UC group and ANCA in other control groups was significant (P <0.05). The value of A in UC group was 80% higher than that in normal control group A value of 2 s or more, and the diagnostic specificity was 89%. Conclusion: There is a diagnostic value of ANCA in UC serum, and neutrophil - ELISA can be used as an important measure of serum ANCA.