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以干木耳子实体为试材,采用常温、4℃、-18℃及液氮等方法对子实体进行保藏,1年后进行组织分离、孢子收集、出耳试验以及酸性蛋白酶、漆酶活性试验,研究保藏方式对木耳子实体的影响。结果表明:耳片组织分离后获得菌丝与继代保藏菌丝无拮抗现象,子实体保藏在菌落形态、菌丝生长速度、分解木屑料能力和酶活性方面均优于继代保藏效果;菌落浓度、漆酶和酸性蛋白酶活性与保藏方式相关性显著,可以作为保藏方式的评价指标,漆酶与菌丝分解木屑速度、催芽率和产量相关性显著,漆酶活性越高,菌丝分解木屑速度越快、催芽率越高、产量越高;无保护剂液氮保藏孢子萌发率最高。木耳子实体保藏简单,并能够更好地保持品种性状,其中无保护剂液氮保藏为最佳保藏方式。
The fruiting bodies of A. lucorum were used as experimental material, and the fruiting bodies were preserved at room temperature, 4 ℃, -18 ℃ and liquid nitrogen, and then separated after 1 year. The spores were harvested, the ears were tested and the activity of acid protease and laccase , To study the impact of preservation methods on the fungus fruiting body. The results showed that there was no antagonism of mycelium and mycelium of the preserved mycelia after isolation of the ear tissue, and the fruiting body was superior to the preservation effect of the subculture in terms of colony morphology, mycelial growth rate, the ability to decompose wood chips and enzyme activity; Laccase and acid protease activity were significantly correlated with the preservation method, which could be used as the evaluation index of the preservation method. The correlation between laccase and mycelia in breaking wood chips speed, germination rate and yield was significant. The higher the laccase activity was, The faster the rate of germination, the higher the rate of production, the higher the yield; unprotected liquid nitrogen storage spores germination rate highest. The fungus fruiting body is easy to preserve and can better maintain the variety traits, of which the unprotected liquid nitrogen is the best preservation way.