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采用生物活性追踪法,将香椿老叶甲醇提取物划分为4个不同极性部位,通过测定其总还原力、1,1-二苯基-2-苦基-肼(DPPH)自由基清除能力和三价铁还原抗氧化能力(FRAP)测试值,确定香椿老叶提取物抗氧化活性及其组成。结果表明:香椿老叶提取物中的强抗氧化活性物质主要集中在乙酸乙酯部位,该部位的总还原力相当于467.53mg/g Vc的总还原值,FRAP值相当于10578μmol/g硫酸亚铁的FRAP值,质量浓度50mg/L的DPPH自由基清除率为92.84%,均比同浓度的2,4-二叔丁基甲基苯酚(BHT)强。相关性研究表明,香椿老叶提取物的抗氧化活性主要在于其含有较高的黄酮类化合物所致。TLC和HPLC分析表明:香椿老叶提取物强抗氧化活性物质主要由4个黄酮-糖苷类化合物组成。
Bioactive tracing method was used to classify the methanolic extracts of old leaves of Toona sinensis into four different polarity fractions. The total reducing power of 1,1-diphenyl-2-picrylhydrazine (DPPH) scavenging ability And ferric iron reduction antioxidant capacity (FRAP) test values to determine the anti-oxidation activity and composition of Chinese toon grass old leaf extract. The results showed that the strong anti-oxidative active substances in the extract of old toona sinensis mainly concentrated in the ethyl acetate fraction, and the total reducing power of this part was equivalent to the total reduction value of 467.53mg / g Vc. The FRAP value was equivalent to 10578μmol / Iron FRAP value, the mass concentration of 50mg / L of DPPH radical scavenging rate of 92.84%, than the same concentration of 2,4-di-tert-butyl methyl phenol (BHT) strong. Correlation studies have shown that the antioxidant activity of Chinese toon extract is mainly due to its high flavonoids. TLC and HPLC analysis showed that the anti-oxidative active substances of the extract of Toona Sinensis mainly consisted of four flavone-glycosides.