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根据已知植物抗病基因的保守区域设计引物 ,从抗稻瘟病水稻品种窄叶青 8号第 1链cDNA和基因组DNA中扩增出 3个与植物抗病基因同源的序列 :Osr1 ,Osr2和Osr3,三者核苷酸水平的同源性在 98%以上 .这 3个基因均含有典型的NBS类抗病基因所拥有的保守结构域 ,如P 环 ,Kinase2a ,Kinase3a以及跨膜结构域等 .Southern杂交分析表明水稻抗病基因同源序列在基因组中以多基因家族的形式存在 ,以窄叶青 8号 /京系 1 7组合构建的DH群体和分子图谱将Osr1基因的 2个拷贝和Osr3基因的 1个拷贝定位在水稻第 1 2染色体上 ,位于 1个抗稻瘟病QTL附近 .Northern杂交结果显示Osr1基因在水稻叶片、幼茎和根中均有转录
Based on the conserved regions of the known plant resistance genes, three primers were designed to amplify the three plant-resistance genes from the first-strand cDNA and genomic DNA of the blast-resistant rice variety Zhongyayuan 8: Osr1, Osr2 And Osr3.The homology of nucleotide level of these three genes is above 98% .These three genes contain the conserved domains of typical NBS type resistance genes such as P-ring, Kinase2a, Kinase3a and transmembrane domain Etc. Southern hybridization analysis showed that the homologous sequences of rice resistance genes exist in the genome as a multi-gene family. The DH population and molecular map constructed by the combination of ZYE8 / Jing-17 combined two copies of Osr1 gene And one copy of Osr3 located on the 12th chromosome of rice in the vicinity of one blast-resistant QTL.The results of Northern hybridization showed that Osr1 gene transcribed in leaves, young stems and roots of rice