对两种草坪草和六种牧草种子生活力四唑测定方法进行了研究，探讨了各种子适宜的预处理方法、四唑溶液浓度和染色时间。结果表明：除春箭舌豌豆种子外，其余种子在染色前均需水中浸渍或缓慢预湿及横切或纵切、针刺等预处理；在３０℃下测定的最佳条件为：假俭草、碱茅和籽粒苋种子用０．１％浓度，染色１４ｈ，狗牙根和串叶松香草种子用０．５％浓度，染色２２ｈ，春箭舌豌豆和湖南稷子种子用０．５％浓度，分别染色１４或６ｈ，小冠花种子用１．０％浓度，染色１４ｈ． The methods for determination of viability of two turf grasses and six species of forage seed were studied, and the suitable pretreatment methods, the concentration of tetrazolium solution and the dyeing time were discussed. The results showed that all the seeds except the pea seeds of spring tongue need to be pretreated by immersion in water, slow pre-wetting, transection or slitting or needle punching before dyeing. The optimum conditions for determination at 30 ℃ are: Grass, Alhummis and Amaricana seeds were stained with 0.1% for 14 h, the roots of C. sinensis and P. xylophilus were stained with 0.5% concentration for 22 h, and the seeds were stained with 0.5% Concentration, respectively, stained 14 or 6h, Corolla seed with 1.0% concentration, stained 14h.