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以吸附的方法将两种具有代表性的含铜氧化酶酪氨酸酶(Tyr)和漆酶(Lac)固定在活性炭粉表面, 结果表明, 活性炭能促进Tyr和Lac的直接电子转移反应, 循环伏安曲线上表现出一对准可逆的氧化还原峰; 式量电位E0’ 几乎不随扫速而变化. 进一步的实验结果显示, 固定在活性炭上的Tyr和Lac能保持对O2还原的电催化作用. 本文固定酶的方法具有简单、易于操作和酶活性保持良好等优点, 可用于获得其他生物氧化还原蛋白质和酶的直接电子转移以及制备生物燃料电池电极催化剂.
The adsorption of two representative copper-containing oxidase tyrosinase (Tyr) and laccase (Lac) on the surface of activated carbon powder, the results show that activated carbon can promote Tyr and Lac direct electron transfer reaction, recycling The voltammogram showed a pair of quasi-reversible redox peaks; the type potential E0 ’hardly changed with the sweep rate.Further experimental results show that Tyr and Lac immobilized on activated carbon can maintain the electrocatalytic effect on O2 reduction The method of immobilization of enzyme in this paper has the advantages of simple, easy operation and good enzyme activity, which can be used to obtain direct electron transfer of other bio-redox proteins and enzymes and prepare biofuel cell electrode catalyst.