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用生物素标记的HBV DNA探针对经83～158周实验的55只树鼩肝组织的福尔马林固定、石蜡包埋的切片进行原位分子杂交研究。在45只接种过人HBV的树鼩中,有30只在肝细胞检出HBV DNA。这55只树鼩,有41只摄入了AFB_1,其中肝细胞HBV DNA阳性的A组22只,有10只发生了HCC;19只HBVDNA阴性的B组,只发生2例HCC,两组差别显著(P<0.05)。未接触过AFB_1的14只树鼩中,肝细胞HBVDNA阳性的8只(C组),发生1例HCC;而HBV DNA阴性的6只(D组)无HCC发生。提示HBV和AFB_1在诱发树鼩HCC中起协同作用;并支持HBV感染与HCC存在病因学关系的观点。该实验HBV DNA阳性物质主要分布于癌外肝细胞胞浆内,少数位于肝细胞核。癌组织内HBV DNA阳性率低于癌外肝组织。与在人肝癌研究的结果一致。表明树鼩是研究HBV致癌分子机理的较好的模型。
Biotin-labeled HBV DNA probes were used to perform in situ molecular hybridization of formalin-fixed and paraffin-embedded sections of 55 tree shrew liver tissues that were tested at 83 to 158 weeks. Of the 45 tree shrews infected with human HBV, 30 were found to have HBV DNA in hepatocytes. Of the 55 tree shrews, 41 had AFB_1, of which 22 were positive for hepatocyte HBV DNA in group A and 10 had HCC; 19 were HBV DNA-negative in group B and only 2 HCCs occurred. Significant (P < 0.05). Among the 14 tree shrews that had not been exposed to AFB_1, 8 were positive for HBV DNA in liver cells (group C), 1 HCC occurred, and 6 HCC groups were negative for HBV DNA (group D). It suggests that HBV and AFB_1 play a synergistic role in the induction of tree shrew HCC; and support the view that HBV infection has an etiological relationship with HCC. The experimental HBV DNA positive substances were mainly distributed in the cytoplasm of extrahepatic hepatocytes, and a few were located in hepatocyte nuclei. The positive rate of HBV DNA in cancer tissue was lower than that in cancerous liver tissue. Consistent with the findings in human liver cancer research. It shows that tree shrew is a better model to study the molecular mechanism of HBV carcinogenesis.