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[目的]为99mTc标记生长抑素类似物奥曲肽对原发性肝癌伴肺转移的诊断与靶向放疗提供实验依据。[方法]用RT-PCR检测高转移潜能的人肝细胞癌株HCCLM6的生长抑素受体2mRNA的表达并测序;建立裸鼠原位肝癌肺转移模型;人工合成99mTc-DOTA-D-Phe1-Tyr3-Octreotide并用于荷瘤裸鼠显像,观察原位肿瘤组织、肺转移瘤及重要器官组织的99mTc-DOTA-D-Phe1-Tyr3-Octreotide摄取分布状况,并比较各组织的摄取比值(T/B)。[结果]RT-PCR和测序结果证实人肝癌细胞株HCCLM6特异高表达生长抑素受体2,扫描结果可见原位肝癌组织、肺转移瘤组织有明显的99mTc-DOTA-D-Phe1-Tyr3-Octreotide摄取,脑、心及正常肺组织等未见明显99mTc-DOTA-D-Phe1-Tyr3-Octreotide聚集,肾脏和膀胱可见明显99mTc-DOTA-D-Phe1-Tyr3-Octreotide放射性浓聚影。[结论]人肝癌细胞株HCCLM6高表达生长抑素受体2,在该受体介导下99mTc-DOTA-D-Phe1-Tyr3-Octreotide能够与原位肝癌及肺转移性肝癌特异结合,可能为放射性核素标记奥曲肽用于原发性肝癌肺转移的诊断及靶向放疗提供一定的理论基础。
[Objective] To provide experimental evidence for the diagnosis and targeted radiotherapy of 99mTc labeled somatostatin analogue octreotide on primary liver cancer with lung metastasis. [Method] The expression of somatostatin receptor 2 mRNA in human hepatocellular carcinoma cell line HCCLM6 with high metastatic potentiality was detected by RT-PCR and sequenced. The lung metastasis model of orthotopic liver cancer in nude mice was established. The 99mTc-DOTA-D-Phe1- Tyr3-Octreotide was used in nude mice to observe the uptake and distribution of 99mTc-DOTA-D-Phe1-Tyr3-Octreotide in tumor tissues, lung metastases and vital organs in nude mice. T / B). [Results] The results of RT-PCR and sequencing confirmed that HCCLM6 specifically expressed somatostatin receptor 2 in human hepatocellular carcinoma cell line HCCLM6. Scanning results showed that 99mTc-DOTA-D-Phe1-Tyr3- There were no significant 99mTc-DOTA-D-Phe1-Tyr3-Octreotide aggregates in Octreotide uptake, brain, heart and normal lung tissues. The 99mTc-DOTA-D-Phe1-Tyr3- [Conclusion] Somatostatin receptor 2 is highly expressed in human hepatocellular carcinoma cell line HCCLM6, and 99mTc-DOTA-D-Phe1-Tyr3-Octreotide can specifically bind to hepatocellular carcinoma and metastatic liver cancer. Radionuclide labeled octreotide for the diagnosis of primary liver cancer lung metastases and targeted radiotherapy provide a theoretical basis.