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为了明确引起方斑东风螺急性死亡症的病原,对2015年6月广东省徐闻县发生的方斑东风螺(Babylonia areolata)急性死亡症进行了病原分离纯化,获得1株优势细菌,命名为XW-01。将XW-01人工感染方斑东风螺,表现出自然发病症状,证实分离菌株为致病菌。分离的菌株经形态学观察、生理生化鉴定和病原16S r RNA序列分析,结果显示该病原菌为哈维氏弧菌(Vibrio harveyi)。XW-01对方斑东风螺半致死剂量(LD50)测定值为6.3×106 cfu/m L。药敏试验结果显示,该病原菌对常见的7种抗菌药物氟哌酸、氟苯尼考、氨苄西林、恩诺沙星、头孢三嗪、左旋氧氟沙星和妥布霉素敏感。添加不同剂量的三联生物噬菌王产品到水族箱中,对方斑东风螺用浸泡法进行人工感染哈维氏弧菌试验,观察东风螺发病及死亡情况。试验结果表明,添加1%的此产品可以明显降低东风螺的死亡率,表明三联生物噬菌王产品对东风螺感染哈维氏弧菌所引起的急性坏死病有明显的预防作用。
In order to clarify the pathogen of the acute death of M. orientalis, the pathogen was isolated and purified from the acute death of Babylonia areolata in Xuwen County, Guangdong Province in June 2015, and one dominant bacterium was obtained, named XW -01. The XW-01 artificial infection of the spot blotch, showing the natural onset of symptoms, confirmed that the isolates as pathogenic bacteria. The isolated strains were identified by morphological observation, physiological and biochemical identification and pathogen 16S rRNA sequence analysis. The results showed that the pathogen was Vibrio harveyi. The XW-01 was found to have a LD50 of 6.3 106 cfu / mL. Susceptibility test results showed that the pathogen was sensitive to seven common antibacterial agents norfloxacin, florfenicol, ampicillin, enrofloxacin, ceftriaxone, levofloxacin and tobramycin. Add different doses of triple biopharmaceutical product to the aquarium, the other spot blotch invasive method for artificial infection with Vibrio harveyi test to observe the incidence and death of Dongfeng snail. The test results showed that adding 1% of this product can significantly reduce the mortality of B. orientalis, indicating that the triple biopharmaceutical product has a significant preventive effect on A. necrosis caused by V. harveyi.