论文部分内容阅读
背景:实验研究发现,羌菖合剂可防治脑供血不足的脑损伤,并可改善血液流变性。目的:研究羌菖合剂对脑供血不足大鼠外周红细胞的作用,探讨改善血液流变性的机制。设计:随机对照实验。单位:成都军区总医院的中心实验室。材料:实验于2003-07/09在成都军区总医院中心实验室完成,选用SD大白鼠50只,雌雄兼用,体质量150～170g。方法:结扎大鼠右侧颈总动脉(CA)造成脑供血不足,并分成对照组和羌菖合剂治疗组,将两组进行对比。结扎CA当天开始,治疗组灌胃给不同剂量羌菖合剂,对照组灌胃给生理盐水。治疗3,7d,仪器分析外周红细胞及其流变性参数。主要观察指标:①实验大鼠红细胞血红蛋白量。②实验大鼠红细胞流变性。结果:①术后3d,对照组外周血平均细胞血红蛋白(MCH)、血球比积(HCT,%)和RBC聚集指数呈增高趋势,平均细胞血红蛋白浓度(MCHC,g/L)明显增大(375±7vs363±13)(P<0.05);羌菖合剂10g/kg治疗组HGB(139.2±12.4vs153.6±9.6),MCHC(355±7vs375±7),HCT(35.4±0.9vs42.8±4.5),显著低于对照组(P<0.05～P<0.001)。②术后7d,对照组外周血RBC刚性指数和聚集指数则呈进一步升高趋势;羌菖合剂10g/kg治疗组MCHC(354±4vs361±4)、RBC刚性指数(1.02±0.35vs4.54±3.04)和聚集指数(2.76±0.66vs4.67±1.21)显著低于对照?
BACKGROUND: Experimental studies have found that Qiangsai Mixture can prevent cerebral insufficiency in brain and improve blood rheology. Objective: To investigate the effect of Qiangsai Mixture on peripheral erythrocytes in rats with cerebral insufficiency and to explore the mechanism of improving hemorrheology. Design: Randomized controlled experiment. Unit: Chengdu Military Region General Hospital Central Laboratory. MATERIALS: Experiments were performed at the Central Laboratory of Chengdu Military Region General Hospital from July to September 09, 2003. Fifty SD rats were selected for both sexes. The body weight was 150-170 g. Methods: Ligation of right common carotid artery (CA) in rats caused cerebral insufficiency and divided into control group and Qiangsai Mixture treatment group. The two groups were compared. Ligation of CA began the same day, the treatment group gavage to different doses of Qiang Chang mixture, the control group gavage with saline. On the 3rd and 7th day after treatment, the peripheral blood erythrocytes and their rheological parameters were analyzed. MAIN OUTCOME MEASURES: ① The amount of erythrocyte hemoglobin in experimental rats. ② Experimental rat erythrocyte rheology. Results: (1) The mean cellular hemoglobin (MCH), hematocrit (HCT,%) and RBC aggregation index in the control group showed a trend of increase after 3 days and the mean cellular hemoglobin concentration (MCHC, g / L) ± 7vs363 ± 13) (P <0.05). HGB (139.2 ± 12.4 vs 153.6 ± 9.6), MCHC (355 ± 7 vs 375 ± 7) and HCT (35.4 ± 0.9 vs 42.8 ± 4.5) in Qiangshen Mixture 10g / ), Significantly lower than the control group (P <0.05 ~ P <0.001). ② RBC rigidity index and aggregation index in the control group increased further on the 7th day after operation. MCHC (354 ± 4vs361 ± 4), RBC rigidity index (1.02 ± 0.35vs4.54 ± 3.04) and aggregation index (2.76 ± 0.66 vs4.67 ± 1.21) were significantly lower than the control?