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目的 探讨建立人皮肤汗腺细胞体外分离及纯化培养的技术。方法 通过酶消化法分离人皮肤汗腺 ,微量加样器在倒置显微镜屏视下吸取游离的汗腺组织移入 Hank平衡盐溶液 (HBSS) ,经纯化后予以原代培养。结果 分离的汗腺可在体外贴壁生长、增殖并传代 ,纯化处理清除了杂质细胞和组织碎片 ,解决了成纤维细胞对汗腺细胞培养的污染难题 ,且汗腺的生长能力无明显改变。结论 人汗腺细胞的培养存在着分离困难和成纤维细胞污染等难题 ,采用本方法可以获得大量处于良好生长状态的高纯度人汗腺细胞。
Objective To explore the establishment of human skin sweat gland cells in vitro isolation and purification of culture technology. Methods Human skin sweat glands were isolated by enzymatic digestion. The microtiter sample was taken in Hank’s Balanced Salt Solution (HBSS) by observing the free sweat gland under the visual microscope screen. The cells were purified and primary cultured. Results The isolated sweat glands could grow, proliferate and pass on in vitro. Purification and removal of impurities cells and tissue fragments solved the problem of fibroblasts pollution on the culture of sweat glands, and the growth capacity of sweat glands did not change significantly. Conclusions Difficulty of separation and fibroblast contamination exist in the culture of human sweat gland cells. A large number of high purity human sweat gland cells can be obtained by this method.