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鸡冠花(Celosia Cristata L.)系苋科一年生草本植物。本实验选用鸡冠花之花序制剂进行。一、鸡冠花注射液的制备:取原药材除去杂质、洗净、晾干、称重,以水提醇沉法,加吐温-80助溶,制成10%鸡冠花注射液。贮藏冰箱中备用。二、取体重30～35g健康小鼠。雌、雄分笼饲养两周。拣出孕鼠,选用未孕鼠。雌、雄以2:1合笼。每天定时检查雌鼠的阴道口有无阴栓形成。将有阴栓之母鼠取出单独饲养,以此作为受孕的第1天。养至第14～16天,即可选用活跃健壮孕鼠做引产实验。将选用之孕鼠随机分成三组。每组10只。甲组为实验组,乙组为已知引产有效药物对照组,丙组为生理盐水对照组。三组均宫腔内注射0.5ml。连续观察48h,记录产出子鼠数量与死亡情况。72h后解剖母鼠,取出未产出的子鼠。结果见表1。
Celosia Cristata L. is an annual herb of the family Polygonaceae. This experiment used the inflorescence preparation of cockscomb. First, the preparation of celosia injection: Take the original herbs to remove impurities, wash, dry, weigh, with water extraction alcohol precipitation method, plus Tween -80 to help dissolve, made of 10% celosia injection. Store in the refrigerator for use. Second, take a weight of 30 ~ 35g healthy mice. Female and male are kept in separate cages for two weeks. Pick out pregnant rats and use unpregnant mice. Females and males use a 2:1 cage. Regularly check the vaginal openings of female mice for the formation of vaginal plugs. Females with female plugs were taken individually and used as the first day of conception. After raising to the 14th to 16th days, active and robust pregnant rats can be selected as induction laboratories. The pregnant rats selected were randomly divided into three groups. 10 in each group. Group A was the experimental group, Group B was the effective drug control group known to induce labor, and Group C was the normal saline control group. Three groups were intrauterine injection of 0.5ml. After 48 hours of continuous observation, the numbers of the offspring and deaths were recorded. After 72 hours, the mother rat was dissected and unproductive mice were removed. The results are shown in Table 1.