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目的研究轻度高温、亚低温对局灶脑缺血组织兴奋性氨基酸(EAA)的影响。方法91只Wistar大鼠按随机数字表法分为:轻度高温、常温、亚低温条件下缺血2h再灌注0,1,2,3h组(按不同脑温分别标志为O2R0,O2R1,O2R2,O2R3组)及假手术组,共13组(n=7);采用大鼠大脑中动脉(MCA)缺血再灌注线栓模型,诱导目标脑温,用HPLC荧光法检测脑组织谷氨酸(Glu)、天冬氨酸(Asp)、甘氨酸(Gly)含量。结果不同时间点轻度高温组Glu,Asp,Gly含量明显高于相应时间点常温组或假手术组(0h:轻度高温组的Glu,Asp,Gly含量分别为(4.3±0.6),(9.4±1.0),(3.4±0.5)μmol/g;常温组分别为(3.0±0.4),(7.0±0.6),(3.0±0.4)μmol/g;假手术组分别为(2.4±0.3),(4.8±0.5),(1.7±0.3)μmol/g;(F=8.17～174.17,P=0.0052～0.0001),不同时间点亚低温组Glu,Asp,Gly含量明显低于相应时间点常温组或轻度高温组(F=5.80～174.17,P=0.0179～0.0001);再灌注后3h内,各脑温组Glu,Asp及亚低温组和常温组Gly均有不同程度下降,但轻度高温组Gly无明显下降,仍高于常温组(F=8.57,P=0.0043)。结论轻度高温促进EAA增高,在持续增加Glu“兴奋毒性”方面起重要作用;亚低温抑制EAA增高,在降低Glu“兴奋毒性”方面起重要作用。
Objective To investigate the effects of mild hypothermia and mild hypothermia on excitatory amino acids (EAA) in focal cerebral ischemia. Methods Totally 91 Wistar rats were randomly divided into three groups according to random number table: mild high temperature, normal temperature and mild hypothermia for 2h and then reperfusion for 0, 1, 2 and 3h groups (according to different brain temperature, O2R0, O2R1 and O2R2 , O2R3 group) and sham-operation group (n = 7). Rat model of cerebral ischemia was induced by reperfusion of middle cerebral artery (MCA) (Glu), aspartic acid (Asp), glycine (Gly) content. Results The contents of Glu, Asp and Gly in mild hyperthermia group at different time points were significantly higher than those in normal temperature group or sham operation group at 0 h (0 h: mild high temperature group, respectively, the contents of Glu, Asp and Gly were (4.3 ± 0.6), (3.0 ± 0.4), (7.0 ± 0.6) and (3.0 ± 0.4) μmol / g respectively in the normal temperature group and (2.4 ± 0.3) and (3.4 ± 0.5) μmol / 4.8 ± 0.5), (1.7 ± 0.3) μmol / g, respectively (F = 8.17-174.17, P = 0.0052-0.0001). The levels of Glu, Asp and Gly in the mild hypothermia group were significantly lower than those in the normal temperature group (F = 5.80-174.17, P = 0.0179-0.0001). Within 3 hours after reperfusion, the levels of Gly in Glu, Asp, mild hypothermia group and normal temperature group in each brain temperature group decreased to some extent, but mild mild hypothermia group Gly (F = 8.57, P = 0.0043) .Conclusion Mild hypothermia can promote the increase of EAA and play an important role in the continuous increase of Glu “excitotoxicity.” Mild hypothermia inhibits the increase of EAA, Excitement "has an important role.