论文部分内容阅读
利用ＤＮＡ重组技术，将分离到的ｂｃｒ／ａｂｌｃＤＮＡ片段克隆到质粒ｐＳＰ７２中。在这个中间克隆载体中，此ｃＤＮＡ片段两端的酶切点已被调整。利用此中间克隆载体和逆转录病毒载体ＢＡＧ，组装了反义ｂｃｒ／ａｂｌＲＮＡ的真核表达载体，此重组表达载体将应用在细胞水平研究反义ＲＮＡ对ｂｃｒ／ａｂｌ基因携带细胞的作用，探索基因治疗。
The isolated bcr / ablcDNA fragment was cloned into plasmid pSP72 using DNA recombination technology. In this intermediate cloning vector, the cleavage sites at both ends of the cDNA fragment have been adjusted. Antisense bcr / ablRNA eukaryotic expression vector was assembled using this intermediate cloning vector and retrovirus vector BAG. The recombinant expression vector will be used to study the effect of antisense RNA on bcr / abl gene-carrying cells at the cellular level to explore the role of genes treatment.