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目的用大孔树脂分离纯化刺五加苷的2种主要成分作为控制刺五加药材质量的标准。方法用D-101大孔树脂对刺五加苷B、刺五加苷E进行分离纯化,用高效液相色谱法测定以上2个成分的含量,以此作为刺五加的质量标准。结果上样液浓度0.5 g·mL-1,吸附流速2 BV·h-1,30%乙醇的洗脱流速1 BV·h-1。透析袋处理。色谱柱:C18(4.6 mm×250 mm,5μm);检测波长220 nm;流动相:水-乙腈(0~10 min,90∶10;10~30 min,90~80∶10~20);流速:1.0 mL·min-1;柱温25℃。结论该方法操作性强,重现性好,可作为刺五加药材质量控制的有效方法。
Objective To separate and purify the two main components of acanthopanax using macroporous resin as the standard to control the quality of acanthopanax medicinal materials. Methods D-101 macroporous resin was used for the separation and purification of acanthopanax B and acanthopanax E. The contents of the above two components were determined by high performance liquid chromatography as the quality standard of Acanthopanax senticosus. Results The sample solution concentration was 0.5 g · mL-1, the flow rate of adsorption was 2 BV · h-1, the flow rate of ethanol was 1 BV · h-1. Dialysis bag treatment. The mobile phase consisted of water-acetonitrile (0-10 min, 90:10; 10-30 min, 90-80:10 ~ 20), and the mobile phase consisted of C18 (4.6 mm × 250 mm, 5 μm) : 1.0 mL · min-1; column temperature 25 ℃. Conclusion The method is robust, reproducible and can be used as an effective method for quality control of Radix.