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以测定的日本对虾肝胰腺细胞的RNA/DNA值作为评价指标,研究了培养基中加入Ca2+和Mg2+对其细胞生长的影响。结果表明,培养基中加入适量的Ca2+和Mg2+可促进肝胰腺细胞的生长,各实验组细胞中的RNA/DNA值均高于对照组;培养基中加入适量的Ca2+和Mg2+的混合液时有助于肝胰腺细胞贴壁生长。
The effect of Ca2 + and Mg2 + on the cell growth was studied by using the measured RNA / DNA value of hepatopancreas cells of Japanese shrimp as evaluation index. The results showed that adding appropriate amounts of Ca2 + and Mg2 + to the medium could promote the growth of hepatopancreas cells, and the RNA / DNA values of the experimental groups were higher than those of the control group. When adding appropriate amount of Ca2 + and Mg2 + Helps to promote the growth of hepatopancreas cells.