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本文应用间接荧光抗体试验比较了４种方法制备的旋毛虫幼虫抗原诊断实验性旋毛虫病的效果。结果表明，旋毛虫纯净幼虫冰冻切片抗原优于完整幼虫抗原、超声粉碎幼虫抗原及膈肌幼虫冰冻切片抗原。用此抗原检测感染旋毛虫的大鼠血清时，抗体阳性率为１００％（１３／１３），而１０份正常大鼠血清均为阴性反应。小鼠感染旋毛虫后２周即可检测到抗旋毛虫抗体，阳性率为１１．１１％（１／９），感染后５周抗体阳性率已升到１００％（８／８），升高的抗体滴度可一直持续至感染后第１５周。此抗原在－２０℃至少可保存２．５年而不丧失其活性和特异性。应用滤纸血也可取得满意的检测结果。
In this paper, indirect fluorescent antibody test was used to compare the efficacy of four methods for diagnosis of Trichinella spiralis by Trichinella spiralis larvae antigen. The results showed that the frozen section antigen of pure Trichinella spiralis larvae was better than that of intact larval antigens, ultrasonic larvae larval antigen and frozen section antigen of diaphragmatic larvae. The anti-Trichinella serovar Typhimurium rat serum was detected by this antigen, the positive rate was 100% (13/13), while 10 normal rat serum were negative. Anti-Trichinella antibodies could be detected 2 weeks after infection with Trichinella in mice, the positive rate was 11.11% (1/9), the positive rate of antibody 5 weeks after infection rose to 100% (8/8) Of the antibody titers can continue until the first 15 weeks after infection. This antigen can be stored at -20 ℃ for at least 2.5 years without losing its activity and specificity. Application of filter paper blood can also be satisfied with the test results.