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应用噻唑蓝法检测从赤魟中分离到的福安泰-03(Fuantai-03,FAT-03)对高转移人卵巢癌细胞HO-8910PM生长的影响;人工重组基底膜检测FAT-03对细胞侵袭能力的影响;SDS-聚丙烯酰胺凝胶电泳检测FAT-03对HO-8910PM细胞分泌MMP-9能力的影响。结果显示,FAT-03(20.0、40.0、60.0μg/ml)作用HO-8910PM细胞24h,对细胞生长的抑制率小于10%,对细胞与纤维粘连蛋白(fibronectin,FN)和Matrigel粘附的抑制率分别为15.1%、28.0%(P<0.05)、54.1%(P<0.01)和14.7%、34.3%(P<0.05)、40.5%(P<0.01),对细胞迁移的抑制率分别为16.4%、33.8%(P<0.05)和42.6%(P<0.01)。此外,FAT-03显著减少HO8910-PM细胞分泌MMP-9的量,并降低其活性。这些实验事实提示FAT-03能明显抑制HO-8910PM细胞的侵袭能力。
The effect of Fuantai-03 (FAT-03) on the growth of highly metastatic human ovarian cancer HO-8910PM cells was detected by the method of thiazolyl blue assay. The effect of FAT-03 on cell invasion was evaluated by artificial recombinant basement membrane The effect of FAT-03 on the secretion of MMP-9 by HO-8910PM cells was detected by SDS-polyacrylamide gel electrophoresis. The results showed that the inhibitory rate of FAT-03 (20.0,40.0,60.0μg / ml) on HO-8910PM cells for 24h was less than 10%, and the inhibition of cell adhesion to fibronectin (FN) and Matrigel The rates of cell migration were 15.1%, 28.0% (P <0.05), 54.1% (P <0.01) and 14.7%, 34.3% (P <0.05) and 40.5% %, 33.8% (P <0.05) and 42.6% (P <0.01) respectively. In addition, FAT-03 significantly reduced the amount of MMP-9 secreted by HO8910-PM cells and decreased their activity. These experimental facts suggest that FAT-03 significantly inhibits the invasiveness of HO-8910PM cells.