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目的：研究 IL-3、GM-CSF能否扩增小鼠骨髓树突状细胞，并通过 MHC Class-I途径提呈外源性抗原。方法：以C57BL/6小鼠骨髓2 h粘附细胞作为树突状细胞来源，在 IL-3（10 ng/ml）及 GM-CSF（1000 U/ml）条件下培养5 d，观察细胞形态、数量和免疫表型的变化，以及对外源性抗原的摄取能力和Class-I途径提呈能力。结果：培养后细胞绝对计数显著增加，MHC分子及共刺激分子表达显著增加，对颗粒性抗原beads-OVA具有高效摄取能力，Class-I途径提呈beads-OVA及SIIFEKL表位的能力显著增强。结论：IL-3、GM-CSF能有效扩增具有高效抗原摄取和提呈能力的树突状细胞，提示在树突状细胞和肿瘤免疫治疗研究中具有重要价值。
Objective: To investigate whether IL-3 and GM-CSF can amplify mouse bone marrow dendritic cells and present exogenous antigens through MHC Class-I pathway. Methods: Bone marrow 2 h adherent cells of C57BL / 6 mice were used as the source of dendritic cells and cultured for 5 days under the conditions of IL-3 (10 ng / ml) and GM-CSF (1000 U / ml) , Number and immunophenotype changes, as well as the ability of exogenous antigen uptake and Class-I pathways. Results: The absolute counts of cells were significantly increased, the expression of MHC molecules and co-stimulatory molecules was significantly increased, and the ability of uptake of granular antigens beads-OVA was high. The ability of Class-I pathway to present beads-OVA and SIIFEKL epitopes was significantly enhanced. CONCLUSION: IL-3 and GM-CSF can effectively amplify dendritic cells with high efficiency of antigen uptake and presentation, suggesting that they are of great value in dendritic cell and tumor immunotherapy.