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通过对草原樱桃与欧洲甜樱桃种间远缘杂交及草原樱桃品种间杂交后花粉管行为的荧光观察发现，草原樱桃品种间杂交授粉后花粉在柱头上能正常萌发生长，花粉管沿花柱到达胚珠完成受精过程，平均座果率为２７％。而草原樱桃与欧洲甜樱桃种间杂交授粉后花粉在柱头上虽少量萌发，但花粉管在柱头上横向生长，或盘绕，扭曲不能伸入花柱；个别进入花柱的花粉管先端因沉积胼胝质而中途停止伸长未能进入子房到达胚珠，从而说明这种杂交授粉后花粉管的不正常行为是导致草原樱桃与欧洲甜樱桃远缘杂交不亲和的主要原因。
Fluorescent observation of the pollen tube behavior in the cross between the savannah and sweet cherry and the cross between the savannah and cherry cultivars showed that the pollens germinated on the stigma after cross pollination, and the pollen tube reached the ovule along the style Completion of the fertilization process, the average fruit set rate of 27%. However, although pollen germinated slightly on the stigma after pollination, the pollen tube grew laterally on the stigma or coiled and distorted and could not reach into the style; Halfway stop elongation failed to enter the ovary to reach the ovule, indicating that pollen tube pollination after this hybridization irregular behavior is the main reason leading to prairie cherry and European sweet cherry distant hybridization incompatibility.