草鱼胸腺细胞培养方法主要有机械分散法和消化分离法。胸腺细胞绝大部分是由大、中、小淋巴细胞组成,而淋巴细胞为悬浮细胞,培养悬浮细胞时由于细胞中期分裂相与培养的细胞不易区分,其传代时间只能凭细胞计数推测其密度来确定是否可以进行细胞传代。在培养草鱼胸腺细胞时,在细胞培养液中加入PHA,观察培养结果可发现能使草鱼胸腺细胞成团生长,增殖旺盛。 Grass carp thymocyte culture methods are mainly mechanical dispersion and digestion. Most of the thymocytes are composed of large, medium and small lymphocytes, while the lymphocytes are suspended cells. When cultured in suspension cells, the metaphase cells are not easily distinguished from the cultured cells, and the passage time can only be estimated by the cell count To determine if cell passage is possible. In the culture of grass carp thymocytes, adding PHA into the cell culture medium, the culture results can be found to make grass carp thymocytes grow into clusters, proliferation strong.