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利用分离自陆稻的稻瘟病菌菌株94-84c(MAT1-1)和95-23-4a(MAT1-2)进行有性杂交,得杂交组合6315;利用本实验室子囊孢子菌株6315R-11(MAT1-1)与中国水稻所提供的6023菌株(MAT1-2)杂交得组合7368;6315R-31(MAT1-1)与95-23-4a(MAT1-2)杂交得组合7370;陆稻分离菌94-80d(MAT1-1)与95-23-4a(MAT1-2)杂交得组合6307。通过6315、7368、7370等3个杂交组合有性世代形成的遗传表现和6307组合色素产生的遗传表现分别对有性世代和色素进行初步的基因表达分析。结果显示,稻瘟病菌的有性世代受2个基因控制,分别定名为PS1基因和PS2基因;色素则仅由一个基因控制,定名为M基因。对有性世代及色素遗传进行深入研究,将为快速、有效地培育抗瘟品种及指导抗侵入杀真菌剂的研制提供理论依据。
Sexual hybridization was carried out using the M. grisea strains 94-84c (MAT1-1) and 95-23-4a (MAT1-2) isolated from Upland rice, resulting in a cross combination 6315; using the Ascosporogenous laboratory Aspergillus sp. Strain 6315R-11 MAT1-1) was crossed with 6023 strain (MAT1-2) provided by Chinese rice to obtain 7,768; combination of 6315R-31 (MAT1-1) with 95-23-4a (MAT1-2) Combination of 94-80d (MAT1-1) with 95-23-4a (MAT1-2) results in combination 6307. The gene expression analysis of sexual generation and pigment were carried out respectively through the genetic expression of 6315, 7368, 7370 and other three hybrid sexual generation and the genetic expression of 6307 combined pigment. The results showed that the sexual generation of Magnaporthe grisea was controlled by two genes named PS1 and PS2, respectively. The pigment was controlled by only one gene and named as M gene. In-depth study of sexual generation and pigment inheritance will provide a theoretical basis for the rapid and effective cultivation of anti-blast varieties and the development of anti-invasive fungicides.