论文部分内容阅读
目的研究建立食品中曲酸的气相色谱分析方法,并应用于样品中曲酸的测定。方法样品经0.01 mol/L盐酸超声提取,一定量提取液采用真空冷冻干燥技术去除水分,残渣用甲醇+乙酸乙酯(1+1)超声溶解,取上清液采用DB-17毛细管柱进行气相色谱分析。结果本方法在2 mg/L~200 mg/L范围内线性良好,相对标准偏差(RSD)0.89%~8.16%(n=6),加标回收率83.5%~104.1%,最低检出限为2.0 mg/L。结论本方法适于果汁、火腿肠等样品中曲酸的测定,方法灵敏度高、结果准确。
Objective To establish a gas chromatographic method for the determination of kojic acid in foods and apply it to the determination of kojic acid in the samples. Methods The samples were extracted with 0.01 mol / L hydrochloric acid. A certain amount of extract was removed by vacuum freeze-drying. The residue was dissolved in methanol and ethyl acetate (1 + 1). The supernatant was collected on a DB-17 capillary column. spectrum analysis. Results The method showed good linearity in the range of 2 mg / L to 200 mg / L with relative standard deviations (RSDs) of 0.89% -8.16% (n = 6) and recoveries of 83.5% -104.1%. The detection limits were 2.0 mg / L. Conclusion The method is suitable for the determination of kojic acid in fruit juice, sausage and other samples. The method has high sensitivity and accurate results.