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目的：动态观察亚急性风寒环境刺激对小鼠免疫器官、Ｔ、Ｂ淋巴细胞免疫功能的影响，并通过观测应激激素、交感肾上腺髓质功能初步探讨其影响的机理。方法：利用人工气候箱制造风寒环境，小鼠置于箱内刺激１０ｈ，于刺激后１ｄ、３ｄ、５ｄ、７ｄ测定其免疫功能，并于刺激后０ｈ、１２ｈ、２４ｈ、３６ｈ测定其应激激素等指标。结果：风寒刺激后，小鼠胸腺萎缩，脾脏减重，胸腺组织ｃＡＭＰ含量及ｃＡＭＰ／ｃＧＭＰ比值升高、外周淋巴细胞ＡＮＡＥ阳性率下降以及脾细胞分裂原反应性降低，免疫抑制高峰发生在刺激后第３天和第５天；同时，血浆Ｃｏｒｔ（皮质酮）、ＮＥ（去甲肾上腺素）、Ｅ（肾上腺素）均一过性大幅升高。结论：风寒二气对机体可构成—强烈应激原，并可经由神经－内分泌－免疫调节网络的相互作用而抑制免疫功能，这应是风寒二气致病机理的一个重要方面。
OBJECTIVE: To observe the effect of sub-acute cold and wind-cold environment on the immune function of immune organs, T lymphocytes and B lymphocytes in mice and to explore the possible mechanism by observing the function of stress hormones and sympathetic adrenal medulla. Methods: The cold environment was made by artificial climate chamber. The mice were placed in the box for 10h, and the immune function was determined on the 1st, 3rd, 5th, 7th day after stimulation. The stress hormones were measured at 0h, 12h, 24h and 36h after stimulation And other indicators. Results: After atrophic stimulation, thymus atrophy, weight loss of spleen, increase of cAMP and cAMP / cGMP in thymus, decrease of ANAE positive rate of peripheral lymphocytes and decrease of splenocyte mitogenic reactivity, immunosuppression peak occurred after stimulation Day 3 and Day 5; at the same time, plasma Cort, NE (norepinephrine), E (epinephrine) were significantly increased. CONCLUSIONS: Cold and Cold Gas can inhibit the immune function through the interaction of neuro-endocrine-immune regulatory network, which may constitute a strong stressor, and this should be an important aspect of the pathogenic mechanism of cold qi.2.