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目的 探讨欧美流行的人类免疫缺陷病毒 (HIV) 1B亚型株与我国HIV感染和艾滋病 (AIDS)病人 gag特异性CD+ 8T细胞应答交叉反应性。方法 研究对象为长期不进展者 (LTNP) 7例和艾滋病患者 9例 ,将覆盖HXB2HIV 1gag全长的 12 5个重叠肽段组成 11个肽段库作为抗原 ,用γ干扰素刺激原酶联免疫斑点试验方法检测LTNP和AIDS病人的特异性CD+ 8T细胞应答 ,观察两组病人间的差异及其与CD+ 4 T细胞和病毒载量的相关性。结果 LTNP组和AIDS组HIV 1gag特异性CD+ 8T细胞应答强度分别为 (12 12± 796 )斑点形成细胞数 (SFC) / 10 6外周血单个核细胞(PBMC)和 (182± 2 0 3)SFC/ 10 6PBMC ,识别肽段库的个数 (间接反应了细胞毒性T淋巴细胞应答的宽度 )分别为 3 0± 0 8和 0 8± 0 7,LTNP组显著高于AIDS组。CD+ 8T细胞应答的强度和宽度与CD+ 4 T细胞计数呈正相关 ,与病毒载量呈负相关。结论 欧美流行株与我国病毒株之间具有交叉反应性 ,HIV 1gag特异性CD+ 8T细胞应答在阻止疾病进展中可能发挥重要作用。
Objective To investigate the cross-reactivity of HIV-1 subtype 1B strains in Europe and the United States with gag-specific CD + 8 T cell responses in HIV-infected and AIDS-infected patients in China. Methods: Seven patients with long-term non-progress (LTNP) and nine patients with AIDS were enrolled in this study. Twelve overlapping peptide fragments covering 1 × 10 6 HXB2HIV were used as antigen. Speckle test was used to detect the specific CD + 8 T cell responses in LTNP and AIDS patients. The differences between the two groups of patients and their relationship with CD + 4 T cell and viral load were observed. Results The response intensities of HIV 1 gag-specific CD + 8 T cells in LTNP group and AIDS group were (12 12 ± 796) SFC / 10 6 peripheral blood mononuclear cells (PBMC) and (182 ± 2 0 3) SFC / 10 6PBMC. The number of peptide libraries (indirectly reflecting the width of cytotoxic T lymphocyte responses) was 30 ± 0 8 and 0 ± 0 7, respectively. The LTNP group was significantly higher than the AIDS group. The intensity and width of CD + 8 T cell responses were positively correlated with CD + 4 T cell counts and negatively correlated with viral load. Conclusion There is cross-reactivity between the EU and US strains and the strain of HIV in our country. The HIV 1gag-specific CD + 8T cell response may play an important role in preventing the progression of the disease.