论文部分内容阅读
目的 :探讨应激对小鼠脑内一氧化氮 (NO)及一氧化氮合酶 (NOS)的影响及意义。方法 :将 6 0只雄性昆明品系小鼠随机分为 3组。其中对照组 2 0只 ,强迫游泳分 2组各 2 0只。分别于强迫游泳 (实验组 )后 1h (一组 )、 2h (二组 )取脑组织检测NO含量及NOS活性。结果 :对照组NO及NOS分别为 2 7 4 7± 15 16 μmol/gprot、 2 16± 0 5 3U/mgprot。实验一组为 17 83± 5 6 3μmpl/gprot、 2 76± 0 87U/mgprot ;实验二组为 11 38± 1 2 2 μmpl/gprot、 3 2 9± 0 4 1U/mgprot。急性应激后 1hNO水平降低并有统计学意义 (t =2 6 7,P <0 0 5 ) ;2h后进一步降低 (t=5 0 5 ,P <0 0 1)。急性应激后 1hNOS活性增高并有统计学意义 (t =-2 4 2 ,P<0 0 5 )。应激后NO与NOS变化呈显著性负相关 (r=-0 316 ,P <0 0 5 )。结论 :神经递质NO及NOS参与了中枢神经急性应激反应 ,且NO水平降低、NOS活性增高。
Objective: To investigate the effect and significance of stress on nitric oxide (NO) and nitric oxide synthase (NOS) in brain of mice. Methods: Sixty male Kunming mice were randomly divided into three groups. 20 in the control group, forced swimming in 2 groups of 20 each. The levels of NO and NOS in brain tissue were measured at 1h (one group) and 2h (two groups) after forced swimming (experimental group) respectively. Results: The NO and NOS in the control group were respectively 2747 ± 1516 μmol / gprot and 216 ± 053U / mgprot. The experimental group was 17 83 ± 5 6 3μmpl / gprot, 2 76 ± 0 87U / mgprot; the second experimental group was 11 38 ± 1 2 2 μmpl / gprot, 3 2 9 ± 0 4 1U / mgprot. The level of NO at 1 hour after acute stress decreased and was statistically significant (t = 267, P <0 05), and further decreased after 2 hours (t = 50 05, P 0 01). After 1 h of acute stress, the activity of NOS increased and was statistically significant (t = -242, P <0 05). There was a significant negative correlation between NO and NOS after stress (r = -0 316, P <0 05). Conclusion: The neurotransmitters NO and NOS are involved in central nervous system acute stress response, and the levels of NO and NOS are increased.